Composite

Part:BBa_K2680104

Designed by: Xiangyi Fang   Group: iGEM18_William_and_Mary   (2018-08-01)


3G J23102

J23102 flanked by prefix sticky end A and suffix sticky end B in a 3G-compatible backbone. Used PCR to add William and Mary Pad overhangs, which were used to clone the part into William and Mary Pad backbone. Forbidden restriction sites were checked for and removed if necessary.

Medium-strong (0.86x that of BBa_J23100) constitutive promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 64
    Illegal NheI site found at 87
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 47
    Illegal BsaI.rc site found at 98


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